Synthetic somatostatin mimics

ABSTRACT

Relatively small cyclic peptides that mimic the biological and/or chemical activity of larger cyclic peptide such as somatostatin, as well as synthetic methods therefor. In certain embodiments, cyclic peptides of the invention have structure: ##STR1## wherein R NP1  is H, an amine protecting group, or a solid support and R NP2  is H or an amine protecting group.

RELATED APPLICATIONS

This application is a continuation of application Ser. No. 08/188,594, filed Jan. 28, 1994, now abandoned, which is a continuation of application Ser. No. 07/938,803, filed Sep. 1, 1992, now abandoned.

FIELD OF THE INVENTION

This invention relates to relatively small cyclic peptides that mimic the biological and/or chemical activity of larger cyclic peptides such as somatostatin.

BACKGROUND OF THE INVENTION

Peptides are implicated in a wide variety of biochemical processes in humans and other mammals. The design of peptide mimics which are resistant to degradation by proteolytic enzymes has become of increasing interest to peptide chemists. A primary goal has been to reduce the susceptibility of mimics to cleavage and inactivation by peptidases.

Somatostatin (SRIF; somatotropin release inhibiting factor), a cyclic tetradecapeptide, was first isolated from bovine hypothalamus and characterized as an inhibitor of growth hormone secretion. SRIF receptors have been found not only on pituitary cells but also in the brain, on gastric or pancreatic cells, and elsewhere. This has stimulated interest in the isolation of SRIF receptors by affinity chromatography in amount sufficient for partial sequence determination. For example, He, et al, Proc. Natl. Acad. Sci. (USA), 1989, 86, 1480, have described the use of D-Trp⁸ SRIF as an affinity ligand.

The bioactive conformation of SRIF is believed to include a β-turn involving residues 7-10 (Phe⁷ -Trp⁸ -Lys⁹ -Thr¹⁰)(SEQ ID NO: 1). These four amino acids have been shown to be necessary and sufficient for receptor recognition and activation, so long as they are held in the proper orientation. Somatostatin accomplishes this proper orientation through its ten remaining amino acids and the cystine bridge contained therein. In a number of active cyclic hexapeptide analogs for somatostatin, proper orientation of the four amino acids is maintained via dipeptide segments. For example, Veber and Hirschmann, et al., Life Sciences, 1984, 34, 1371 and Nature, 1981, 292 have reported that cyclic hexapeptides typified by cyclo(Pro-Phe-D-Trp-Lys-Thr-Phe) are potent mimics of somatostatin.

OBJECTS OF THE INVENTION

It is one object of the present invention to provide cyclic peptides that mimic the biological and/or chemical activity of larger cyclic peptides.

It is another object to provide cyclic peptides that are chemically more stable than naturally-occurring cyclic peptides, particularly under conditions such as found in the human body.

It is yet another object to provide cyclic peptides that can be used as affinity ligands for G-protein-linked receptors, particularly the somatostatin receptor.

It is a further object of the invention to provide simple yet efficient methods for synthesizing cyclic peptides.

SUMMARY OF THE INVENTION

These and other objects are accomplished by the present invention, which provides relatively stable, cyclic peptides that bind G-protein linked receptors such as the somatostatin receptor.

In certain embodiments, cyclic peptides of the invention have structure (1): ##STR2## wherein: R₁, R₂, R₃, R_(5A), and R₆ are, independently, (CH₂)_(x) -R_(x) where x is 0 to about 5 and R_(x) is:

alkyl or alkenyl having from about 1 to about 5 carbon atoms, from 0 to about 3 oxygen atoms, from 0 to about 3 nitrogen atoms, and from 0 to about 3 sulfur atoms; and

aryl having 3 to about 14 carbon atoms, from 0 to about 4 oxygen atoms, from 0 to about 4 nitrogen atoms, and from 0 to about 4 sulfur atoms; or

NHR_(NP2) where R_(NP2) is H or an amine protecting group;

R_(4A) is alkyl or alkenyl having from about 1 to about 14 carbon atoms;

R_(NP1) is H, an amine protecting group, or a solid support; and

R₇ is H or alkyl having about 1 to about 3 carbon atoms; or

R₆ and R₇ together form a heterocyclic ring having about 4 to about 8 atoms.

In other embodiments, the cyclic peptides of the invention have structure (2): ##STR3## wherein: R₁, R₂, R₃, R_(4B), and R₆ are, independently, (CH₂)_(x) -R_(x) where x is 0 to about 5 and R_(x) is:

alkyl or alkenyl having from about 1 to about 5 carbon atoms, from 0 to about 3 oxygen atoms, from 0 to about 3 nitrogen atoms, and from 0 to about 3 sulfur atoms; and

aryl having about 3 to about 14 carbon atoms, from 0 to about 4 oxygen atoms, from 0 to about 4 nitrogen atoms, and from 0 to about 4 sulfur atoms; or

NHR_(NP2) where R_(NP2) is H or an amine protecting group;

R_(5B) is alkyl or alkenyl having from about 1 to about 14 carbon atoms;

R_(NP1) is H, an amine protecting group, or a solid support; and

R₇ is H or alkyl having about 1 to about 3 carbon atoms; or

R₆ and R₇ together form a heterocyclic ring having about 4 to about 8 atoms.

The present invention also provides synthetic methods for these and other cyclic peptides, as well as intermediates useful in such methods. For example, the invention provides methods for preparing cyclic peptides having structure (3): ##STR4## wherein: R₁ -R₆ are, independently, (CH₂)_(x) -R_(x) where x is 0 to about 5, and R_(x) is:

alkyl or alkenyl having from about 1 to about 5 carbon atoms, from 0 to about 3 oxygen atoms, from 0 to about 3 nitrogen atoms, and from 0 to about 3 sulfur atoms; and

aryl having about 3 to about 14 carbon atoms, from 0 to about 4 oxygen atoms, from 0 to about 4 nitrogen atoms, and from 0 to about 4 sulfur atoms; or

NHR_(NP2) where R_(NP2) is H or an amine protecting group;

at least one of R₄ -R₆ is R_(Q) -NH-R_(NP1) wherein R_(Q) is alkyl or alkenyl having from about 1 to about 14 carbon atoms and R_(NP1) is H; and

R₇ is H or alkyl having about 1 to about 3 carbon atoms; or

R₆ and R₇ together form a heterocyclic ring having about 4 to about 8 atoms;

These methods comprise the steps of providing a first compound having structure (4): ##STR5## wherein R_(NP1), R_(NP2), and R_(NP3) are amine protecting groups, R_(CP) is a carboxyl protecting group, and R_(NP3) and R_(CP) can be removed in the presence of R_(NP1) and R_(NP2). In accordance with the invention, compounds having structure (4) are cyclized and exposed to conditions effective to remove the R_(NP1) group. Cyclic peptides thus prepared can be attached to a solid support via the resultant free amine group.

DETAILED DESCRIPTION OF THE INVENTION

In preferred embodiments, cyclic peptides according to the present invention have structure (1) or structure (2): ##STR6## wherein: R₁, R₂, R₃, R_(4B), R_(5A), and R₆ are, independently, (CH₂)_(x) -R_(x) where x is 0 to about 5 and R_(x) is:

alkyl or alkenyl having from about 1 to about 5 carbon atoms, from 0 to about 3 oxygen atoms, from 0 to about 3 nitrogen atoms, and from 0 to about 3 sulfur atoms; and

aryl having about 3 to about 14 carbon atoms, from 0 to about 4 oxygen atoms, from 0 to about 4 nitrogen atoms, and from 0 to about 4 sulfur atoms; or

NHR_(NP2) where R_(NP2) is H or an amine protecting group;

R_(4A) and R_(5B) are alkyl or alkenyl having from about 1 to about 14 carbon atoms;

R_(NP1) is H, an amine protecting group, or a solid support; and

R₇ is H or alkyl having about 1 to about 3 carbon atoms; or

R₆ and R₇ together form a heterocyclic ring having about 4 to about 8 atoms.

Alkyl groups of the invention include but are not limited to straight chain, branched chain, and cyclic hydrocarbons such as methyl, ethyl, propyl, butyl, pentyl, isopropyl, 2-butyl, isobutyl, 2-methylbutyl, and isopentyl moieties, as well as heteroatomic derivatives thereof. Alkenyl groups include but are not limited to unsaturated moieties derived from such alkyl groups, including but not limited to vinyl and allyl moieties. Aryl groups include but are not limited to aromatic and heteroaromatic moieties such as phenyl, tolyl, benzyl, naphthyl, anthracyl, phenanthryl, xylyl, hydroxyphenyl, indole, imidazole, tetrazole, and triazole moieties, including halogenated derivatives thereof. Suitable heterocyclic groups include but are not limited to pyrrolidine and piperidine moieties.

Protecting groups are known per se as chemical functional groups that can be selectively appended to and removed from functionality, such as amine groups, present in a chemical compound to render such functionality inert to chemical reaction conditions to which the compound is exposed. See. e.g., Greene and Wuts, Protective Groups in Organic Synthesis, 2d edition, John Wiley & Sons, New York, 1991. Numerous amine protecting groups are known in the art, including the benzyloxycarbonyl (CBz), chlorobenzyloxycarbonyl, t-butyloxycarbonyl (Boc), fluorenylmethoxycarbonyl (Fmoc), isonicotinyloxycarbonyl (i-Noc) groups. (see. e.g., Veber and Hirschmann, et al., J. Org. Chem., 1977, 42, 3286 and Atherton, et al., The Peptides, Gross and Meienhofer, Eds, Academic Press; New York, 1983; Vol. 9 pp. 1-38). For example, it is known that the Boc group can protect an amine group from base and from reducing conditions but that it can be removed with acid.

The cyclic peptides of the present invention can be attached to a solid support for use as, for example, affinity ligands. Such attachment preferably is effected through a free amine group such as the amine group appended to the R_(4A) group of structure (1) or the amine group appended to the R_(5B) group of structure (2). Suitable solid supports are well known to persons skilled in the arts of chromatography and/or solid phase synthesis. (See. e.g., He, et al., Proc. Natl. Acad. Sci. (USA), 1989, 86, 1480; Cuatrecasas, et al., Proc. Natl. Acad. Sci. (USA), 1968, 61, 636; and Bethell, et al., J. Biol. Chem., 1979, 259, 2572.) Useful supports include PAM resins, Merrifield peptide resins, and polyamide peptide resins. A preferred solid support is AFFI-GEL 10, which is commercially available from Bio-Rad (Richmond, Calif.).

Preferred cyclic peptides of the invention are those wherein R₁ -R₇ are derived from the side chains of naturally-occurring amino acids. Exemplary side chains are provided in Table 1.

                                      TABLE 1                                      __________________________________________________________________________     CH.sub.3           CH.sub.3 CH.sub.2SCH.sub.2CH.sub.2HOCH.sub.2CH.sub.2        HOCH.sub.2         CH.sub.3 CH(OH)                                             C.sub.6 H.sub.5CH.sub.2                                                                           HO.sub.2 CCH.sub.2NH.sub.2 C(O)CH.sub.2                     HOC.sub.6 H.sub.5CH.sub.2                                                                          ##STR7##                                                    ##STR8##          HO.sub.2 CCH.sub.2CH.sub.2                                   ##STR9##          NH.sub.2 C(O)CH.sub.2CH.sub.2 (CH.sub.3).sub.2CH                               (CH.sub.3).sub.2CHCH.sub.2 CH.sub.3CH.sub.2CH.sub.2                            H.sub.2 NCH.sub.2CH.sub.2CH.sub.2                            ##STR10##         H.sub.2 NC(NH)NHCH.sub.2CH.sub.2CH.sub.2 H.sub.2                               NC(O)NHCH.sub.2CH.sub.2CH.sub.2 CH.sub.3 CH.sub.2CH(CH.                        sub.3) CH.sub.3CH.sub.2CH.sub.2CH.sub.2                     HSCH.sub.2         H.sub.2 NCH.sub.2CH.sub.2CH.sub.2CH.sub.2                   HO.sub.2 CCH(NH.sub.2)CH.sub.2SSCH.sub.2                                       CH.sub.3 CH.sub.2                                                              CH.sub.3SCH.sub.2CH.sub.2                                                      __________________________________________________________________________

Preferred cyclic peptides are those wherein R₁ is hydroxybenzyl, R₂ is --CH₂ --(indole), and/or R₃ is --(CH₂)₄ NHR_(NP2), wherein R_(NP2) is H or an Fmoc group. Cyclic peptides having structures (5) and (6) are particularly preferred. ##STR11## Preferably, R_(4A) is --(CH₂)₃ NH--, R_(5B) is --(CH₂)₄ NH--, and R_(NP1) is H or an isonicotinyloxycarbonyl protecting group. Cyclic peptides having structure (7) or structure (8) are particularly preferred. ##STR12##

These and other cyclic peptides are prepared through cyclization of suitably protected linear peptides such as structures (9) and (10): ##STR13## wherein R_(CP) is a carboxyl protecting group, R_(HP) is a hydroxyl protecting group, and R_(NP1), R_(NP2), and R_(NP3) are amine protecting groups. For example, cyclic peptides having structures (1) and (2) can be prepared by processes that include cyclizing synthetic intermediates (11) and (12), respectively. ##STR14## Carboxyl protecting groups are known per se as chemical functional groups that can be selectively appended to and removed from carboxyl functionality present in a chemical compound to render such functionality inert to chemical reaction conditions to which the compound is exposed. Numerous carboxyl protecting groups are known in the art, including trimethylsilyl, 2-(trimethylsilyl)ethyl, 2,2,2-trichloroethyl, t-butyldimethylsilyl, p-methoxybenzyl, t-butyl, and 2-methylthioethyl groups. (See. e.g., Greene and Wuts, Protective Groups in Organic Synthesis, 2d edition, John Wiley & Sons, New York, 1991, pp. 224-276.) By analogy, hydroxyl protecting groups can be appended to and removed from hydroxyl groups. Representative hydroxyl groups include 2-chlorobenzyl, 2,6-dichlorobenzyl, cyclohexyl, t-butyl, o-nitrobenzyl, methylthiomethyl, allyl, t-butyldimethylsilyl, and t-butyldiphenylsilyl groups. (See, e.g., Greene and Wuts, Protective Groups in Organic Synthesis, 2d edition, John Wiley & Sons, New York, 1991, pp. 1-179.)

The respective amine, acid, and hydroxyl protecting groups should be carefully selected such that hydroxyl protecting group R_(HP), carboxyl protecting group R_(CP), and amine protecting group R_(NP3) can be removed in the presence of amine protecting groups R_(NP1) and R_(NP2). Also, it should be possible to remove the R_(NP1) group in the presence of the R_(NP2) group. In preferred embodiments, R_(HP), and R_(CP), are acid labile, R_(NP1) is stable to acid but labile under reducing conditions, and R_(NP2) is stable to acid and reducing conditions but labile to base. More preferably, R_(HP) is 2-chlorobenzyl, R_(CP) is 2-(trimethylsilyl)ethyl, R_(NP3) is a Boc group, R_(NP1) is an i-Noc group, and R_(NP3) is a Fmoc group.

Additional objects, advantages, and novel features of this invention will become apparent to those skilled in the art upon examination of the following examples thereof, which are not intended to be limiting.

EXAMPLE 1

Linear peptides having structures (11) and (12) R_(CP) =2-(trimethylsilyl)ethyl, R_(HP) =2-chlorobenzyl, R_(NP1) =i-Noc, R_(NP2) =Fmoc, R_(NP3) =Boc! were synthesized by solution chemistry from N.sup.α Boc-O-2-chlorobenzyl-Tyr Boc-Tyr(Cl-Bzl! generally in accordance with Mendelson, et al., J. Org. Chem. 1983, 48, 4127. The latter was converted into the corresponding 2-(trimethylsilyl)ethyl ester. In elaborating the linear peptide, the N.sup.α -Boc amino acids were coupled using N,N'-dicyclohexylcarbodiimide and 1-hydroxybenzotriazole in methylene chloride in the presence of N-methyl morpholine generally in accordance with Bosshard, et al., Helv. Chim. Acta., 1973, 56, 717. N,N-Bis(2-oxo-3-oxazolidinyl)phosphinic chloride was used as a coupling reagent when the N-Me-Phe was involved in amide bond formation. (see, e.g., Tung, et al., J. Amer. Chem. Soc., 1985, 107, 4342; van der Auwera, et al., Int. J. Peptide Res., 1987, 29, 574 and Int. J. Peptide Res. 1987, 29, 464). Boc groups were removed with TFA generally in accordance with Masui, et al., Bull. Chem. Soc. Jpn., 1980, 53, 464.

N.sup.α -Boc-N.sup.δ -iNoc-Orn, N.sup.α -Boc-N^(e) -iNoc-Lys¹¹ and N.sup.α -Boc-N.sup.ε -Fmoc-Lys were prepared from the corresponding N.sup.α -Boc amino acids using procedures described in the literature. (see, e.g., Veber and Hirschmann, et al., J. Org. Chem. 1977, 42, 3286; Bosshard, et al., Helv. Chim. Acta., 1973, 56, 717). N-Tetrabutyl ammonium fluoride could not be employed for the selective removal of the 2-(trimethylsilyl)ethyl ester because it is sufficiently basic to cleave the Fmoc group at Lys. Further, both the HF-pyridine complex and HF in acetonitrile failed to remove the ester protecting group.

Methanesulfonic acid in TFA removed the C-terminal ester, the N-terminal Boc group as well as the 2-chlorobenzyl ether to afford linear peptides having structures (11) and (12) R_(CP) =R_(HP) R_(NP3) =H, R_(NP1) =i-Noc, R_(NP2) =Fmoc!. (see, e.g., Yajima, et al., The Peptides, Gross and Meienhofer, Eds, Academic Press; New York, 1983; Vol. 5 pp. 65-109 and references cited therein). The crude material was directly cyclized using diphenylphosphoryl azide (DPPA) and solid NaHCO₃ in anhydrous dimethylformamide (DMF) under high dilution conditions, yielding the cyclic peptides having structures (7) and (8) R_(NP1) =i-Noc, R_(NP2) =Fmoc!. (see, e.g., Brady, et al., J. Org. Chem. 1987, 52, 764). Aliquots of the crude materials and final products were purified by reverse phase C-18 HPLC and fully characterized by 500 MHz NMR, amino acid analyses, and FAB Mass Spectroscopy.

Catalytic hydrogenolysis with 10% palladium on carbon cleaved the i-Noc protecting groups. After purification by reverse phase HPLC, the cyclic peptides were coupled to an Affi-gel 10 (Bio-Rad) affinity gel generally in accordance with He, et al, Proc. Natl. Acad. Sci. (USA), 1989, 86, 1480. To free the ε-amino group of Lys⁹, the affinity gel column was washed with piperidine in DMF. That this procedure was effective could be demonstrated by monitoring the effluents by UV.

EXAMPLE 2

The procedure of Example 1 was repeated, except that structures (7) and (8) were treated with piperidine in methylene chloride to cleave the Fmoc groups.

EXAMPLE 3

Using a procedure generally in accordance with Raynor, et al, J. Pharmacol. Exp. Ther., 1989, 251, 510, the compounds produced in Examples 1 and 2 completely displaced ¹²⁵ I! CGP 23996 (des-Ala¹, Gly² -desamino-Cys³ Tyr¹¹ ! dicarba³,14 somatostatin) from SRIF receptors on membranes of AtT-20 cells with IC₅₀ values of 30.1, 2.6, 3.8 and 2.5 nM respectively. By comparison, MK-678 (see, e.g., Veber and Hirschmann, et al., Life Sciences, 1984, 34, 1371) had an IC₅₀ value of 4.4 nM in this assay.

Those skilled in the art will appreciate that numerous changes and modifications may be made to the preferred embodiments of the invention and that such changes and modifications may be made without departing from the spirit of the invention. For example, the strategy described herein may be useful for the isolation of somatostatin receptors and may also be useful for the selective attachment of other ligands to affinity gels. It is therefore intended that the appended claims cover all such equivalent variations as fall within the true spirit and scope of the invention.

    __________________________________________________________________________     SEQUENCE LISTING                                                               (1) GENERAL INFORMATION:                                                       (iii) NUMBER OF SEQUENCES: 5                                                   (2) INFORMATION FOR SEQ ID NO:1:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 4                                                                  (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:                                        PheTrpLysThr                                                                   (2) INFORMATION FOR SEQ ID NO:2:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 6 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: circular                                                         (ii) MOLECULE TYPE: peptide                                                    (ix) FEATURE:                                                                  (A) NAME/KEY: Modified-site                                                    (B) LOCATION: 2-3, Xaa at Line 72                                              (D) OTHER INFORMATION: /label=protecting1                                      /notes-"an amine protecting group or a solid                                   support can be present or absent on the primary                                amino group", Xaa=Orn                                                          (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:                                        TrpLysXaaPheProTyr                                                             15                                                                             (2) INFORMATION FOR SEQ ID NO:3:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 6                                                                  (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (ix) FEATURE:                                                                  (A) NAME/KEY: Modified-site                                                    (B) LOCATION: 1-3                                                              (D) OTHER INFORMATION: /label=protecting1                                      /note="an amine protecting group or a solid                                    support can be present or absent on the primary                                amino group", Xaa: =Orn                                                        (ix) FEATURE:                                                                  (A) NAME/KEY: Modified-site                                                    (B) LOCATION: 6, Xaa at Line 100                                               (D) OTHER INFORMATION: /label=protecting2                                      /note="a carboxyl protecting group can be                                      present or absent on the hydroxyl group", Xaa=Orn                              (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:                                        TrpLysXaaPheProTyr                                                             15                                                                             (2) INFORMATION FOR SEQ ID NO:4:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 6                                                                  (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (ix) FEATURE:                                                                  (A) NAME/KEY: Modified-site                                                    (B) LOCATION: 1,2,4                                                            (D) OTHER INFORMATION: /label=protecting1                                      /note="an amine protecting group or a solid                                    support can be present or absent on the primary                                amino group"                                                                   (ix) FEATURE:                                                                  (A) NAME/KEY: Modified-site                                                    (B) LOCATION: 5                                                                (D) OTHER INFORMATION: /label=methyl                                           /note="a methyl group is present on the amino                                  group"                                                                         (ix) FEATURE:                                                                  (A) NAME/KEY: Modified-site                                                    (B) LOCATION: 6                                                                (D) OTHER INFORMATION: /label=protecting3                                      /notes="a hydroxyl protecting group can be                                     present or absent on the hydroxyl group"                                       (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:                                        TrpLysValLysPheTyr                                                             15                                                                             (2) INFORMATION FOR SEQ ID NO:5:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 6                                                                  (B) TYPE: amino acid                                                           (C) TOPOLOGY: circular                                                         (ii) MOLECULE TYPE: peptide                                                    (ix) FEATURE:                                                                  (A) NAME/KEY: Modified-site                                                    (B) LOCATION: 2, 4                                                             (D) OTHER INFORMATION: /label=protecting1                                      /note="an amine protecting group or a solid                                    support can be present or absent on the primary                                amino group"                                                                   (ix) FEATURE:                                                                  (A) NAME/KEY: Modified-site                                                    (B) LOCATION: 5                                                                (D) OTHER INFORMATION: /label=methyl                                           /note="a methyl group is present on the amino                                  group"                                                                         (ix) FEATURE:                                                                  (A) NAME/KEY: Modified-site                                                    (B) LOCATION: 6                                                                (D) OTHER INFORMATION: /label=protecting3                                      /note="a hydroxyl protecting group can be                                      present or absent on the hydroxyl group"                                       (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:                                        TrpLysValLysPheTyr                                                             15                                                                             __________________________________________________________________________ 

What is claimed is:
 1. A compound having the structure: ##STR15## wherein: R₁ is hydroxybenzyl;R₂ is --CH₂ --(indole); R₃ is --(CH₂)₄ NHR_(NP2), where R_(NP2) is H or an amine protecting group; R_(4B) is (CH₂)_(x) -R_(x) where x is 0 to 5 and R_(x) is alkyl having from 1 to 5 carbon atoms, from 0 to 3 oxygen atoms, from 0 to 3 nitrogen atoms, and from 0 to 3 sulfur atoms; R_(5B) is alkyl having from 1 to 14 carbon atoms; R_(NP1) is H, an amine protecting group, or a solid support; and R₆ is (CH₂)_(y) -R_(y) where y is 0 to 5 and R_(y) is aryl having 3 to 14 carbon atoms, from 0 to 4 oxygen atoms, from 0 to 4 nitrogen atoms, and from 0 to 4 sulfur atoms; and R₇ is alkyl having 1 to about 3 carbon atoms.
 2. The compound of claim 1 wherein R_(4B) is (CH₂)--CH₃, R_(5B) is C₄ alkyl, R₆ is C₆ aryl, and R₇ is C₁ alkyl.
 3. The compound of claim 1 wherein R_(4B) is (CH₂)--CH₃.
 4. The compound of claim 1 wherein R_(5B) is C₄ alkyl.
 5. The compound of claim 1 wherein R₆ is C₆ aryl.
 6. The compound of claim 1 wherein R₇ is C₁ alkyl.
 7. The compound of claim 1 wherein R_(NP2) is H or a fluorenylmethoxycarbonyl protecting group.
 8. The compound of claim 1 wherein R_(5B) is --(CH₂)₃ --.
 9. The compound of claim 8 wherein R_(NP1) is H or an isonicotinyloxycarbonyl protecting group.
 10. The compound of claim 1 wherein R₇ is methyl.
 11. The compound of claim 1 having structure: ##STR16##
 12. The compound of claim 1 having structure (SEQ ID NO: 5): ##STR17## wherein R_(NP1) is H, an amine protecting group, or a solid support and R_(NP2) is H or an amine protecting group.
 13. The compound of claim 1 wherein R_(NP2) is a fluorenylmethoxycarbonyl protecting group.
 14. The compound of claim 1 wherein R_(NP1) is an isonicotinyloxycarbonyl protecting group.
 15. A compound having the structure: ##STR18## wherein: R_(NP3) is H or an amine protecting group;R_(CP) is H or a carboxyl protecting group; R₁ is hydroxybenzyl; R₂ is --CH₂ --(indole); R₃ is --(CH₂)₄ NHR_(NP2), where R_(NP2) is H or an amine protecting group; R_(4B) is (CH₂)_(x) -R_(x) where x is 0 to 5 and R_(x) is alkyl having from 1 to 5 carbon atoms, from 0 to 3 oxygen atoms, from 0 to 3 nitrogen atoms, and from 0 to 3 sulfur atoms; R_(5B) is alkyl having from 1 to 14 carbon atoms; R_(NP1) is H, an amine protecting group, or a solid support; and R₆ is (CH₂)_(y) -R_(y) where y is 0 to 5 and R_(y) is aryl having 3 to 14 carbon atoms, from 0 to 4 oxygen atoms, from 0 to 4 nitrogen atoms, and from 0 to 4 sulfur atoms; and R₇ is alkyl having 1 to about 3 carbon atoms.
 16. The compound of claim 15 wherein R_(4B) is (CH₂)--CH₃, R_(5B) is C₄ alkyl, R₆ is C₆ aryl, and R₇ is C₁ alkyl.
 17. The compound of claim 15 wherein R_(4B) is (CH₂)--CH₃.
 18. The compound of claim 15 wherein R_(5B) is C₄ alkyl.
 19. The compound of claim 15 wherein R₆ is C₆ aryl.
 20. The compound of claim 15 wherein R₇ is C₁ alkyl.
 21. The compound of claim 15 wherein R_(NP3) is a t-butoxycarbonyl protecting group.
 22. The compound of claim 15 wherein R_(CP) is a 2-(trimethylsilyl) protecting group.
 23. The compound of claim 15 wherein R_(5B) is --(CH₂)₃ --. 